This project presents a theoretical framework of online medical laboratory system, it discussed about different scholar’s perspective of online laboratory system, the aim of this project to develop an online medical Laboratory booking system for patients to make lab booking at their convenient time because currently, Bliz Hospital uses a manual based system to keep record of customer’s booking and make new laboratory bookings. This project discussed the tool and technology used in developing the proposed system (the system has a front end by using html, Css, JavaScript to display the content structure and a back end of database using MySQL and PHP). Laboratory systems were compared to identify their similarities and differences. A number of development methodologies were discussed and why one of the methodologies was chosen for this project. Methods used to gather the requirement specification was also discussed and how the researcher will use this as a guideline in developing the proposed system. Diagrams were created describing each system. Demonstration of different WebPages available on the website was discussed and then an evaluation was conducting using two methods to evaluate the website. Online medical laboratory system will enable patients book the medical laboratory test in an hospital